KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Anti-CD206 | Biorad | RRID:AB_493697 |
| Human Anti-CD163 Biotin | Biolegend | RRID:AB_1134004 |
| Mouse Anti-CD45 Biotin | Biolegend | RRID:AB_312968 |
| Anti-CD11b Alexa 647 | Biolegend | RRID:AB_389327 |
| Human TruStain FcX™ | Biolegend | Cat: 422301 |
| Mouse Anti-CD16/32 | Biolegend | RRID:AB_312800 |
| Mouse Anti-CD45 BV510 | Biolegend | RRID:AB_2561392 |
| Mouse Anti-CD3 PE-Cy7 | Biolegend | RRID:AB_1732068 |
| Mouse Anti-C11b BV711 | Biolegend | RRID:AB_11218791 |
| Mouse Anti-Ly6C Alexa-Fluor 700 | BD Biosciences | RRID:AB_10612017 |
| Mouse Anti-Ly6G Percp–cy5.5 | Biolegend | RRID:AB_1877272 |
| Mouse Anti-CD4 BUV395 | BD Biosciences | RRID:AB_2739427 |
| Mouse Anti-CD8–BV605 | Biolegend | RRID:AB_2561352 |
| Anti-pimonidazole GFP | Hypoxyprobe | HP6-1000Kit |
| Mouse Anti-Foxp3 Efluor 450 | Fisher | RRID:AB_1518812 |
| Human Anti-TMEM119 OPAL 520 | Millipore Sigma | RRID:AB_2681645 |
| Human Anti-Sox2 OPAL 620 | Abcam | RRID:AB_10585428 |
| Human Anti-CA9 OPAL 540 | Novus Biologics | RRID:AB_11011250 |
| Human Anti CD163 OPAL 520 | Abcam | RRID:AB_2753196 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| lentiCRISPR v2 | Addgene | RRID:Addgene_52961 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| RGX-202-01 compound | Inspirna | N/A |
| L-ARGININE:HCL (13C6, 99%) | Cambridge isotope | CLM-2265-H-PK |
| 1C13 labeled creatine (guanidino group) | Cambridge isotope | CLM-7933-PK |
| Dialyzed FBS | Fisher | A3382001 |
| SILAC RPMI 1640 Flex Media, no glucose, no phenol red | Fisher | A2494201 |
| DMEM, no Glucose, phenol, glutamine, pyruvate | Fisher | A1443001 |
| Recombinant M-CSF | Peprotech | 315-02 |
| Streptavidin MicroBeads | Miltenyi | 130-048-101 |
| DMEM/F12 | Fisher | 11320033 |
| Recombinant murine EGF | R&D | 2028-EG-200 |
| Recombinant murine FGF | R&D | 3139-FB-025 |
| B27 supplement | Fisher | 17504044 |
| Culture-Insert 2 Well in μ-Dish 35 mm | Ibidi | 81176 |
| Pimonidazole | Hypoxyprobe | HP6-1000Kit |
| Live/Dead Elfuor780 | Fisher | 65-0865-14 |
| StemPro™ Accutase™ Cell Dissociation Reagent | Fisher | A1110501 |
| Critical Commercial Assays | ||
| CellTiter-Glo® Luminescent Cell Viability Assay | Promega | G7570 |
| Adult Brain Dissociation Kit | Miltenyi | 130-107-677 |
| CyQUANT™ MTT Cell Viability Assay | Fisher | V13154 |
| RNeasy Plus Micro Kit | Qiagen | 74034 |
| Deposited Data | ||
| Raw and Analyzed data (that is not included in Data S1) | Upon Request | |
| Raw data/gels/Wes data used to generate figures | Data S1 | |
| Metabolomics Datasets | Metabolomics Workbench | doi: 10.21228/M8PH9P Study: ST002935 |
| Murine scRNA seq data | Biosample submission portal/NIH | BioProject ID: PRJNA1029174 |
| Experimental Models: Cell Lines | ||
| Murine glioma GL-261 Sex: M |
NCI | DTP, DCTD TUMOR REPOSITORY RRID:CVCL_Y003 |
| Murine glioma CT-2A Sex: M |
Millipore | RRID:CVCL_ZJ44 |
| Murine Glioma: GSC-005 Sex: Unknown |
Verma Laboratory | N/A |
| Human GBM cell line: U251 Sex: M |
ATCC | RRID:CVCL_0021 |
| Human GBM PDX: GBM39 Sex: Unknown |
James Laboratory | N/A |
| Experimental Models: Organisms/Strains | ||
| MOUSE: C57 Bl/6 | Jackson | Cat#000664; RRID:IMSR_JAX:000664 |
| MOUSE: LysM-CRE | Jackson | RRID:IMSR_JAX:004781 |
| MOUSE: GATM-Flox | Spiegelman Laboratory | N/A |
| MOUSE: LysM-CRE x GATM-Flox | This Paper | N/A |
| Oligonucleotides | ||
| β-Actin Forward: TTGCTGACAGGATGCAGAAG | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| β-Actin Reverse: ACATCTGCTGGAAGGTGGAC | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| Slc6a8 Forward: AGACTTGGACACGCCAGATG | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| Slc6a8 Reverse: TTCCTCCAACCAGGGCAATC | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| Mouse Hif1a Forward: CTATGGAGGCCAGAAGAGGGTAT | Purchased: IDT | PMID: 22930750 |
| Mouse Hif1a Reverse: CCCACATCAGGTGGCTCATAA | Purchased: IDT | PMID: 22930750 |
| Mouse Hif2a Forward: CTGAGGAAGGAGAAATCCCGT | Purchased: IDT | PMID: 22930750 |
| Mouse Hif2a Reverse: TGTGTCCGAAGGAAGCTGATG | Purchased: IDT | PMID: 22930750 |
| Human Hif1a Forward: CATAAAGTCTGCAACATGGAAGGT | Purchased: IDT | PMID: 18393939 |
| Human Hif1a Reverse: ATTTGATGGGTGAGGAATGGGTT | Purchased: IDT | PMID: 18393939 |
| Human Hif2a Forward: GCGCTAGACTCCGAGAACAT | Purchased: IDT | PMID: 18393939 |
| Human Hif2a Reverse: TGGCCACTTACTACCTGACCCTT | Purchased: IDT | PMID: 18393939 |
| Slc6a8 Reverse: TTCCTCCAACCAGGGCAATC | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| Gatm Forward: GCTTCCTCCCGAAATTCCTGT | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| Gatm Reverse: CCTCTAAAGGGTCCCATTCGT | Purchased: IDT | Design: ncbi.nlm.nih.gov/tools/primer-blast/ |
| SiRNA oligos (full list in Data S1) | Purchased: Sigma | |
| Recombinant DNA | ||
| Software and Algorithms | ||
| Prism | Graphpad | www.graphpad.com; RRID:SCR_002798 |
| Facs Diva | BD | www.bdbiosciences.com |
| Excel | Microsoft | www.microsoft.com; RRID:SCR_016137 |
| FlowJo | Treestar | www.flowjo.com; RRID:SCR_008520 |
| ImageJ | NIH | imagej.nih.gov/ij/; RRID:SCR_003070 |
| Other | ||