a–c, Locomotor activity in untethered, C57BL6/J mice during 15 min following vehicle or drug treatment and 45 min following amphetamine treatment (see Fig. 2a). Effects of haloperidol, olanzapine, clozapine, or MP-10 (a), xanomeline, VU0467154, or SEP-363856 (b), and SKF39393, SCH23390, or SCH39166 (c) on baseline and amphetamine-driven locomotion. The ‘low’ and ‘high’ doses we subsequently used for Ca2+ imaging experiments are indicated in red. Data are expressed as mean ± s.e.m. (****P < 10−4 ***P < 10−3, **P < 10−2 and *P < 0.05 for comparison to vehicle treatment; ####P < 10−4 and ##P < 10−2 compared to vehicle + amphetamine treatment; One-way ANOVA with Holm-Sidak’s multiple comparison test).