Fig. 3. TIF-IA augments transcription of a Pol I reporter gene. HeLa and NIH 3T3 cells were transfected with 10 µg of pHr-CBH or pMr1930-BH and increasing amounts of pCMV-FLAG-hTIF-IA. Transcripts from the reporter plasmid were visualized on northern blots. To normalize for variations in RNA loading, the filter was hybridized with a riboprobe against cytochrome c oxidase.