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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Nov 11;42(11):113427. doi: 10.1016/j.celrep.2023.113427

Figure 4. Inactivating HMCES self-reversal delays DPC resolution in cells.

Figure 4.

(A) Immunoblot of U2OS or HMCESΔ cells complemented with WT HMCES, E127Q HMCES, or EV.

(B) Quantification of HMCES-DPC levels in untreated cells that express WT or E127Q HMCES. RADAR assay was performed with samples from S-phase synchronized cells. Mean ± SEM, n = 4, two-tailed t test.

(C) Representative image of RADAR assay to detect HMCES-DPC from WT- or E127Q-HMCES-expressing cells.

(D) RADAR assay of HMCES-DPC levels from WT- or E127Q-HMCES-expressing cells. Mean ± SEM, n = 4.

(E) Quantification of HMCES-DPC levels with time zero immediately after CD437 set at 100%. Mean ± SEM, n = 4, two-way ANOVA.