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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Nov 11;42(11):113427. doi: 10.1016/j.celrep.2023.113427

Figure 5. HMCES self-reversal is important for cell fitness and responses to DNA damage.

Figure 5.

(A) U2OS cells and HMCESΔ cells expressing an EV or near-endogenous levels of WT or E127Q HMCES were plated at equal cell numbers. Cell proliferation/viability was measured using alamarBlue 5 days later. Mean ± SEM, n = 6, one-way ANOVA with Dunnett post-test.

(B) HMCESΔ cells were infected with retroviruses to overexpress the indicated HMCES WT and mutant proteins. Cells were selected for 3 days with puromycin and then plated at equal cell numbers. Viable cells were counted using trypan blue staining at each time point. Mean ± SEM, n = 3.

(C) Percentage of the viability of the indicated cells treated with CD437 measured using alamarBlue 4 days after a 24 h exposure to drug. Two-way ANOVA, n = 3. WT vs. E127Q, ***p < 0.0004, ****p < 0.0001.

(D) Percentage of viability of the indicated cells treated with KBrO3 as measured using alamarBlue 3 days after a 48 h exposure to drug. Two-way ANOVA, n = 3. WT vs. E127Q, ****p < 0.0001.