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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Cell Rep. 2023 Nov 11;42(11):113427. doi: 10.1016/j.celrep.2023.113427

Figure 6. Model of CD437-induced HMCES-DPC reversal and repair.

Figure 6.

Our data are consistent with recent biochemical studies showing that the thiazolidine linkage is reversible.14,21,22 This linkage was initially thought to be highly stable because it appeared unchanged in biochemical reactions even days after formation, could be observed by crystallography, and blocked the action of AP endonucleases.7 However, the addition of a second ssDNA-AP oligonucleotide trap revealed that HMCES could move to another substrate,21 indicating the DPC formation is reversible and regenerates an intact HMCES protein.