| Antibodies |
|
| Rabbit polyclonal anti HMCES |
Sigma |
Cat#HPA044968; RRID:AB_2679160 |
| Mouse anti-DVC1 (SPRTN) |
John Rouse lab |
N/A |
| Mouse anti ssDNA |
DSHB |
AB_10805144 |
| Goat Anti-Rabbit IgG (H + L) |
Fisher Scientific |
Cat#111-035-144; RRID:AB_2307391 |
| StarBright Blue 700 Goat Anti-Mouse IgG |
Bio-Rad |
Cat#12004158; RRID:AB_2884948 |
|
| Chemicals, peptides, and recombinant proteins |
|
| RLT plus buffer |
Qiagen |
Cat#1053393 |
| IdU |
Sigma Aldrich |
Cat#I7125 |
| KBrO3
|
Acros |
Cat#446941000 |
| MG132 |
Sigma Aldrich |
Cat#M7449 |
| TAK243 |
Chemietek |
Cat#AOB 87172 |
| CD437 |
Tocris bioscience |
Cat#1549 |
| Uracil-DNA Glycosylase (UDG) |
NEB |
Cat#M0280S |
| alamarBlue |
ThermoFisher |
Cat#DAL1100 |
| Aldehyde reactive probe |
Dojindo |
Cat#A305 |
| Wild-type HMCES SRAP protein |
This study |
N/A |
| E127Q HMCES SRAP protein |
This study |
N/A |
|
| Critical commercial assays |
|
| Comet assay kit |
Tevigen |
Cat#4250-050-K |
|
| Experimental models: Cell lines |
|
| U2OS |
ATCC |
Cat#HTB-96; RRID:CVCL_0042 |
| HCT-116 |
ATCC |
Cat#CRL-11268; RRID:CVCL_1926 |
|
| Oligonucleotides |
|
| SPRTN siRNA |
Dharmacon |
J-015442-19-0002 |
| SPRTN siRNA |
Dharmacon |
J-015442-20-0002 |
| SPRTN siRNA |
Dharmacon |
J-015442-21-0002 |
| SPRTN siRNA |
Dharmacon |
J-015442-22-0002 |
| 20mer = d(TCTTCTGGTCUGGATGGTAGT) |
IDT |
N/A |
| 40mer = d(GGAATCTGACTCTTCTGGTCUGGATGGTAGTTAAGTCTTGT) |
IDT |
N/A |
| C40mer = d(ACAAGACTTAACTACCATCCAGACCAGAAGAGTCAGATTCC) |
IDT |
N/A |
| T40mer control = d(GGAATCTGACTCTTCTGGTCTGGATGGTAGTTAAGTCTTGT) |
IDT |
N/A |
|
| Recombinant DNA |
|
| pLPG-HMCES (pKM354) |
This manuscript |
N/A |
| pLPG-E127Q HMCES (pJRF27) |
This manuscript |
N/A |
| pLPCX-EV (pJRF32) |
This manuscript |
N/A |
| pLPCX-OE WT HMCES (pJRF01) |
This manuscript |
N/A |
| pLPCX-OE E127Q HMCES (pJRF33) |
This manuscript |
N/A |
| pLPCX-OE C2A HMCES (pDC1351) |
This manuscript |
N/A |
| pLPCX-OE C2A/E127Q HMCES (pDC1352) |
This manuscript |
N/A |
|
| Software and algorithms |
|
| Graphpad Prism |
Graphpad Software |
https://www.graphpad.com/scientific-software/prism/; RRID: SCR_000306 |
| Image Lab |
Bio-Rad |
https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z
|
