FIG. 5.

Functional analysis of the nnp-1 promoter. (A) Schematic representation of the nnp-1 promoter and the derived reporter constructs. The positions of the shifted E-boxes are indicated by vertical grey bars. (B) Binding of dMyc to the nnp-1 promoter. Chromatin recovered from naive S2 cells or from S2 cells overexpressing HA-dMyc was analyzed by ChIP using an anti-HA antiserum. DNA was amplified with primer pairs located near the E-box (E) (indicated by a thick black line in panel A) or at a distance (U) (see the supplemental material). (C and D) Normalized luciferase activities of the indicated reporter constructs at 24 h (C) or 60 h (D) after transfection. −, gfp, dmyc, and dmnt indicate the cotransfected dsRNA. Three independent experiments were performed, each in triplicate, and gave similar results. Results of a representative experiment are shown. Error bars, standard errors of the means.