. Author manuscript; available in PMC: 2025 Jan 1.

Published in final edited form as: Mol Microbiol. 2023 Dec 1;121(1):116–128. doi: 10.1111/mmi.15202

Table 2.

Primers used in this study. Lower case letters indicate att sites.

Purpose/Name	Sequence (5’−3’)
*Cloning*
*ΔpqsA primers:*
attB1-pqsA-UpF	ggggacaagtttgtacaaaaaagcaggcttcCTACGAAGCCCGTGG
attB2-pqsA- DownR	ggggaccactttgtacaagaaagctgggtaCCGAGGACCTTCTGCAAC
PAO1^ΔPf4 *primers:*
PfiT-DownF	TGATGGCTTTCTACTCCTGA
attB2-PfiT-DownR	ggggaccactttgtacaagaaagctgggtaAGCCGCTCAACCCGATCTA
PfiT seq F	CCACACGTTCGCCAGTCACTT
PfiT seq R	AATGCCGGCCACTTCATCGAC
Pf4-UpF-GWL	tacaaaaaagcaggctTCTGGGAATACGACGGGGGC
Pf4-UpR-GM	tcagagcgcttttgaagctaattcgGATCCCAATGCAAAAGCCCC
Pf4-DnF-GM	aggaacttcaagatccccaattcgCGTCATGAGCTTGGGAAGCT
Pf4-DnR-GWR	tacaagaaagctgggtTGGCAGCAGACCCAGGACGC
pf4-out F	AGTGGCGGTTATCGGATGAC
pf4-out R	TCATTGGGAGGCGCTTTCAT
*qPCR primers:*
AttR-F	taggcatttcaggggcttgg
AttL-R	gagctacggagtaagacgcc
Pf4-RF gBlock	GGGGACAAGTTTGTACAAAAAAGCAGGCTTCTAGGCATTTCAGGGGCTTGGCAGGGTGATTTGGAGCGGGCGAAGGGAATCGAACCCTCGTCATGAGCTTGGGAAGCTCAGGTAATGCTAAAATAGGGTTTTGAAGCGTTCCTATACATTCTAATGCCACTGCCTTCGATTTTTAGGCGTCTTACTCCGTAGCTCTACCCAGCTTTCTTGTACAAAGTGGTCCCC