FIG. 2.

TbLRTP is associated with the basal body. (A) Intact trypanosomal cytoskeletons were prepared by extraction with 1% Nonidet P-40 as described in Materials and Methods. Fractions that were soluble in 1% Nonidet P-40 (S) and insoluble fractions (cytoskeletons) (P) were immunoblotted with anti-TbLRTP, anti-TbBiP, or anti-tubulin antibodies. (B) Immunofluorescence analysis of Nonidet P-40-extracted and methanol-fixed PCF cells showing a close association of TbLRTP signal (red) and the basal body (BB) revealed using BBA4 antibodies (green). (C) Immunolocalization in isolated flagellum preparations from wild-type PCF trypanosomes of TbLRTP (red; left panels), BBA4 (green; middle panel, top), and PFR (green; middle panel, top) and merged images (right panels) show that TbLRTP is localized distally to the basal body within the flagellum. Phase-contrast images are overlaid in all panels. Bars, 5 μm.