FIG. 4.
TbLRTP functions in basal body division and new flagellum biogenesis. The effect of overexpression or p2T7Ti/LRTP PCF trypanosomes induced for 3 days (RNAi) on components of the microtubule cytoskeleton in PCF cells was investigated using immunofluorescence microscopy. Cells were stained for α-tubulin (Tub), basal bodies (BB), and PFR (MAb) in green. All cells were counterstained with DAPI, which is shown in a merge with the phase-contrast image. TbLRTP overexpression resulted in increased cell size. TbLRTP RNAi knockdown resulted in smaller cells that often contained increased numbers of flagella and FAZ elements. Bars, 5 μm.