FIG. 6.

The TbLRTP orthologue of Leishmania is differentially expressed during the life cycle. Western blot of protein lysates from procyclic (P) (flagellated, noninfective, proliferative form), metacyclic (M) (flagellated, infective, nonproliferative form), and amastigote (nonflagellated infective, proliferative form) L. mexicana probed with antibodies against TbLRTP or, as a loading control, N-myristoyl transferase (LmNMT) (29). The positions of molecular weight markers (in thousands) are indicated. Anti-TbLRTP defines a protein in proliferative, procyclic lysates whose migration is consistent with the predicted molecular mass of 53.8 kDa, which we designate LmLRTP. Expression of LmLRTP is severely down-regulated in quiescent metacyclics and replicating, aflagellate amastigotes.