TABLE 1.
Nuclear/kinetoplast analysis of TbLRTP mutantsa
| Strainb | % of total cell population in each strain with the following karyotypec:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1K1N | 2K1N | 2K2N | AK | >2N | 0N | >2K | 0K | 1K2N | |
| WT | 78.5 | 9.1 | 11.4 | 1.0 | 0 | 0 | 0 | 0 | 1 |
| ++ | 50.4 | 11.3 | 9.8 | 28.5 | 5.3 | 0.7 | 0.7 | 9.0 | 12.8 |
| RNAi | 57.3 | 18.3 | 10.7 | 13.7 | 3.1 | 0 | 3.7 | 0.8 | 6.1 |
a
Nuclear/kinetoplast classification was performed by immunofluorescence assays following DAPI staining to determine both nuclear and kinetoplast copy numbers.
b
WT, wild type; ++, TbLRTP overexpresser; RNAi, p2T7Ti/LRTP PCF trypanosomes induced for 3 days. More than 130 cells were analyzed for each strain.
c
AK, all cells with abnormal karyotypes, i.e., not 1K1N, 2K1N, or 2K2N. Percentages in the four columns on the left add up to 100%; the five rightmost columns provide a breakdown of the percentages in the AK column.