KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-SERPINB3 | R & D Systems | Cat# MAB6528; RRID:AB_2943627 |
| Rabbit polyclonal anti-SERPINB3 | Thermo Fisher Scientific | Cat# PA5-30164; RRID:AB_2547638 |
| Mouse monoclonal anti-c-Myc | MilliporeSigma | Cat# M4439; RRID:AB_439694 |
| Mouse monoclonal anti-BBOX1 | MilliporeSigma | Cat# WH0008424M1; RRID:AB_1840194 |
| Mouse monoclonal anti-β-actin | MilliporeSigma | Cat# A5441; RRID:AB_476744 |
| Mouse monoclonal anti-Cas9 | Cell Signaling Technology, Inc. | Cat# 14697; RRID:AB_2750916 |
| Rabbit polyclonal anti-SERPINB3 | Abcam | Cat# ab154971; RRID:AB_2943626 |
| Rabbit polyclonal anti-CD31 | Abcam | Cat# ab28364; RRID:AB_726362 |
| Mouse monoclonal anti-8-hydroxy-2’-deoxyguanosine | Abcam | Cat# ab48508; RRID:AB_867461 |
| Bacterial and virus strains | ||
| DH5α Competent Cells for Subcloning | Thermo Fisher Scientific | Cat# EC0111 |
| Biological samples | ||
| Pancreatic tumor tissues from resected PDAC patients | University of Maryland Medical System (UMMS) in Baltimore, MD University Medical Center Göttingen, Germany | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| L-carnitine | MilliporeSigma | Cat# C0158 |
| Hydroxyproline | MilliporeSigma | Cat# H5534 |
| Gemcitabine hydrochloride | MilliporeSigma | Cat# G6423 |
| Palmitic acid | MilliporeSigma | Cat# P5585 |
| Bovine Serum Albumin | MilliporeSigma | Cat# A7030 |
| cOmplete™ Protease Inhibitor Cocktail | MilliporeSigma | Cat# 4693116001 |
| Calpeptin | MedChemExpress LLC | Cat# HY-100223 |
| RPMI 1640 Medium, GlutaMAX™ Supplement | Thermo Fisher Scientific | Cat# 61870127 |
| IMDM | Thermo Fisher Scientific | Cat# 12440053 |
| McCoy’s 5A (Modified) Medium | Thermo Fisher Scientific | Cat# 16600082 |
| RIPA Lysis and Extraction Buffer | Thermo Fisher Scientific | Cat# 89901 |
| SuperBlock™ Blocking Buffer | Thermo Fisher Scientific | Cat# 37515 |
| SuperSignal™ West Dura Extended Duration Substrate | Thermo Fisher Scientific | Cat# 34075 |
| TRIzol™ Reagent | Thermo Fisher Scientific | Cat# 15596018 |
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | Cat# 4368813 |
| TaqMan™ Fast Advanced Master Mix for qPCR | Thermo Fisher Scientific | Cat# 4444557 |
| Puromycin Dihydrochloride | Thermo Fisher Scientific | Cat# A1113803 |
| Corning® Matrigel® Basement Membrane Matrix, LDEV-free | Corning | Cat# 354234 |
| Target Retrieval Solution, pH 9 | Agilent Technologies | Cat# S2367 |
| Liquid DAB+ | Agilent Technologies | Cat# K346811-2 |
| RNeasy Micro Kit | QIAGEN | Cat# 74004 |
| RNeasy Plus Mini Kit | QIAGEN | Cat# 74134 |
| QIAfilter Plasmid Midi Kit | QIAGEN | Cat# 12243 |
| DNeasy Blood & Tissue Kit | QIAGEN | Cat# 69506 |
| Critical commercial assays | ||
| Calpain-Glo™ Protease Assay | Promega | Cat# G8502 |
| Cell Counting Kit-8 | Dojindo | Cat# CK04 |
| Pierce™ BCA Protein Assay Kits | Thermo Fisher Scientific | Cat# 23225 |
| CellROX™ Deep Red | Thermo Fisher Scientific | Cat# C10422 |
| MitoTracker™ Red FM | Thermo Fisher Scientific | Cat# M22425 |
| MitoSOX™ Red | Thermo Fisher Scientific | Cat# M36008 |
| Deposited data | ||
| Raw RNA sequencing data | This paper | NCBI GEO: GSE223909. This SuperSeries is composed of the following SubSeries GSE224564; GSE223908; GSE223536 |
| Raw metabolome data | This paper | https://osf.io/3jyut/?view_only=fed315a5a79f4cc283790eefc73fca3f |
| Experimental models: Cell lines | ||
| AsPC-1 | American type culture collection (ATCC) | RRID:CVCL_0152 |
| BxPC-3 | American type culture collection (ATCC) | RRID:CVCL_0186 |
| Capan-1 | American type culture collection (ATCC) | RRID:CVCL_0237 |
| Capan-2 | American type culture collection (ATCC) | RRID:CVCL_0026 |
| CFPAC-1 | American type culture collection (ATCC) | RRID:CVCL_1119 |
| MIA PaCa-2 | American type culture collection (ATCC) | RRID:CVCL_0428 |
| PANC-1 | American type culture collection (ATCC) | RRID:CVCL_0480 |
| Panc 10.05 | American type culture collection (ATCC) | RRID:CVCL_1639 |
| SU.86.86 | American type culture collection (ATCC) | RRID:CVCL_3881 |
| 293T | American type culture collection (ATCC) | RRID:CVCL_0063 |
| Experimental models: Organisms/strains | ||
| NOD-SCID mouse | Jackson Laboratory | RRID:IMSR_JAX:005557 |
| Oligonucleotides | ||
| All the oligonucleotides can be found in Table S7 | This paper | N/A |
| Recombinant DNA | ||
| Empty vector control | Genecopoeia | Cat# EX-NEG-Lv103 |
| SERPINB3 vector | Genecopoeia | Cat# EX-F0390-Lv103 |
| Software and algorithms | ||
| MetaboAnalyst 5.0 | Pang et al.53 | RRID:SCR_015539 |
| GraphPad Prism 9 | GraphPad Software | RRID:SCR_002798 |
| CFX384 Touch Real-Time PCR Detection System | Bio-Rad Laboratories, Inc. | RRID:SCR_018057 |
| FlowJo version 10.8.1 | Becton, Dickinson and Company | RRID:SCR_008520 |
| Integrated Genomics Viewer (IGV) | Robinson et al.54 | RRID:SCR_011793 |
| Ingenuity Pathway Analysis | QIAGEN | RRID:SCR_008653 |
| Partek Genomics Suite | Partek Inc. | RRID:SCR_011860 |