FIG. 5.

Expression of human β-like globin genes in the yolk sac of mutDR TgM. Semiquantitative RT-PCR analysis of β-like globin gene expression. Total RNA was prepared from the yolk sac of two embryos (9.5 dpc) derived from the intercross of male transgenic and female wild-type animals. Samples were collected from two independent litters from each mutant line. Expression of human ɛ (hɛ)- and human γ (hγ)-globin compared to endogenous mouse α (mα)-globin genes was analyzed separately by semiquantitative RT-PCR. The signals for hɛ-globin at 18 cycles and hγ/mα-globin at 12 cycles were quantified by PhosphorImager, and the ratios of hɛ/mα (top) and hγ/mα (bottom) were calculated (the mouse α signal at 12 cycles was set at 100% and the values are normalized by transgene copy numbers). The average ± standard deviation from at least three independent experiments was calculated and graphically depicted. Representative results are shown below each panel.