FIG. 7.

Expression of human β-like globin genes in the fetal liver of mutCCTTG TgM. (A) Semiquantitative RT-PCR analysis of β-like globin gene expression. Total RNA was prepared from the livers of two fetuses (14.5 dpc) in two independent litters (lit.1 and lit.2) for each line. The average and standard deviation from at least three independent experiments are graphically depicted. Representative results are shown below each panel. See the legend to Fig. 4 for details. (B and C) RT-PCR analysis of human Gγ/Aγ transcription ratios in the fetal liver. (B) PstI digestion of RT-PCR products. See the legend to Fig. 3 for details. (C) The contribution of the Gγ and Aγ genes relative to total γ-globin synthesis are quantified from panel B and plotted as hatched and solid bars, respectively. Average values obtained from two independent experiments are shown.