FIG. 8.

Expression of human β-like globin genes in the yolk sac of mutCCTTG TgM. Semiquantitative RT-PCR analysis of β-like globin gene expression. Total RNA was prepared from the yolk sac of two embryos (9.5 dpc) derived from the intercross of male transgenic and female wild-type animals. Samples were collected from two independent litters (lit.1 and lit.2) for each mutant line. The average ± standard deviation was calculated (n = 3) and is graphically depicted. Representative results are shown below each panel. See the legend to Fig. 5 for details.