FIG. 1.
FAT10 and a FAT10 conjugate are rapidly degraded in a proteasome-dependent manner. The tetracycline-inducible FAT10 transfectant TB1N was labeled with [35S]Met-Cys and chased for the indicated time periods in the absence (A) or presence (C) of the proteasome inhibitor lactacystin (80 μM) followed by immunoprecipitation against HA-FAT10. Lanes 1 in panels A and C represent noninduced TB1N cells (n.i.). The bands shown in panels A and C were quantified on a radioimager and plotted in panels B and D, respectively, as percent radioactivity based on values obtained after the pulse.