FIG. 2.
Deletion of Spt3p or subunits required for SAGA integrity, but not Gcn5p, impairs recruitment of SAGA by Gcn4p. (A) ChIP analysis of a gcn4Δ SPT7-myc strain (gcn4Δ) and GCN4 SPT7-myc strains containing WT SAGA subunits or the indicated SAGA subunit deletions and harboring high-copy-number GCN4-HA plasmid pHQ1239 was carried out as described in Fig. 1 using anti-myc antibodies. (B) Same as panel A except that ADA2-myc strains were analyzed. (C) Same as panel A except that TRA1-FL strains were analyzed and anti-Flag M2 antibodies were used. The ratio %IP (GCN4/gcn4Δ) values as defined in Fig. 1 were calculated for the ARG1UAS, SNZ1UAS, and ARG4 probes, and the average results obtained from two or more independent cultures and two or more PCR amplifications for each culture were plotted in the histograms with standard errors shown as error bars. The numbers under the histograms, corresponding to percentages of the WT Gcn4p-dependent binding of myc-Spt7p, myc-Ada2p, or FL-Tra1p, were calculated by subtracting unity from all ratio %IP (GCN4/gcn4Δ) values for each mutant and expressing the result as a percentage of the corresponding WT value.