FIG. 3.

Western analysis of tagged SAGA or mediator subunits in coactivator mutants. (A-D) myc- or Flag-tagged strains used in Fig. 2 or 5 or the untagged parent strain BY4741 (panel C) was grown under the same conditions used for ChIP analysis, and WCEs were prepared and subjected to Western blot analysis using anti-myc, anti-Flag M2, or anti-Gcd6p antibodies, the last serving as loading control. The Western signals obtained using the ECL chemiluminescence kit (Amersham) were quantified by video densitometry using NIH image software, and the ratios of myc-Spt7p, myc-Ada2p, myc-Srb6p, or myc-Gal11p to Gcd6p signals are listed for each mutant relative to the WT strain between the two blots.