FIG. 4.

Expression of the human CtIP restored proliferation of NIH 3T3 cells with mCtip knocked down. a, Human GFP-CtIP fusion protein stably expressed in NIH 3T3 cells is resistant to Ctip-siRNA depletion. A stable clone of NIH 3T3 cells expressing human GFP-CtIP fusion protein was established and infected with Ctip-siRNA adenoviruses. Cell lysates prepared from the infected cells were analyzed by Western blotting probed with anti-CtIP and anti-p84 antibodies. b. The stable clone of NIH 3T3 cells was labeled with BrdU after infection with 10 MOI of GFP or Ctip-siRNA for different time intervals, and the results of BrdU incorporation are summarized.