FIG. 5.
Ctip-mediated G1 control is Rb dependent. a, Immunofluorescence staining of BrdU uptake in the pair of Rb+/+ and Rb−/− MEFs (×400) infected with Ctip-siRNA adenovirus or in the other pair of human osteosarcoma cells (×200), Saos-2, and SR5, transiently transfected with pCtIP-RNAi plasmids. Cells were labeled with BrdU 48 h after infection or transfection. b, Summary of BrdU incorporation of Rb+/+ and Rb−/− MEFs at 48, 60, and 72 h after infection. c, The infected Rb+/+ and Rb−/− MEFs were collected for Western blotting probed with antibodies against CtIP, Rb, p21, and actin. d, Summary of the BrdU-positive cells of Saos-2 and SR5, after transfection with either pCtIP-RNAi plasmid for 48 or 72 h.