The α1 helix of RAP74 is important for phosphodiester bond completion and NTP-driven translocation. Model independent analyses comparing TFIIF 74(1-227) (left panels A, C, E, G, and I) and TFIIF 74(1-158) (right panels B, D, F, H, and J). Note the differences in timescales (x-axes; times in seconds). Reactions containing α-amanitin (α-A) in panels I and J are indicated (open triangles).