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Global kinetic analyses of RNAP II elongation stimulated by TFIIF 74(1-227) and TFIIF 74(1-158). Kinetic data are shown for a range of GTP concentrations (μM). Solid red symbols indicate HCl quench data; open symbols indicate EDTA quench data. Residuals (differences between the kinetic simulation and the data points) are indicated beneath each graph. Data were fit to the kinetic models shown in Fig. 7B and C by using the program DYNAFIT (25).
