FIG. 2.
p38 MAPK contributes to the regulation of c-Jun expression in response to UV. Wild-type (wt) (A and C) and jnk−/− (B and C) MEFs without treatment (−) or pretreated (+) with SB203580 (SB; 10 μM) were UV radiated (60 J/m2) and incubated for the times indicated. (A and B) Total RNA was extracted, and the amounts of c-jun mRNA were measured by quantitative PCR. Results are expressed as increases relative to the mRNA extracted from unstimulated fibroblasts ± standard errors of duplicate samples. (C) MEF extracts were analyzed for c-Jun and MAPKAPK2 expression by immunoblot analysis using specific polyclonal anti-c-Jun and anti-MAPKAPK2 antibodies. The detection of tubulin expression was performed to monitor protein loading. The figure is representative of three independent experiments.