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. 2005 May;25(9):3519–3534. doi: 10.1128/MCB.25.9.3519-3534.2005

FIG. 6.

FIG. 6.

Elucidation of the domains of CKIP-1 required for interaction with CP. CKIP-1 deletions were generated and tested for interaction with CP by immunoprecipitation. (A) Schematic representation of the Flag-tagged CKIP-1 deletions generated. aa, amino acids. (B) Flag-tagged CKIP-1 deletions were transfected into U2-OS cells, and the lysates derived from these cells were separated by SDS-PAGE and transferred to PVDF membranes for immunoblotting (IB) with anti-Flag M2 antibody. (C) CKIP-1 deletions were transfected into U2-OS cells and immunoprecipitated (IP) with anti-Flag M2 antibodies. Immunoprecipitates were separated by SDS-PAGE and transferred to PVDF membranes for immunoblotting with anti-CPβ antibody. Interactions between CKIP-1 and CP require amino acids 133 to 193. HC and LC, IgG heavy and light chains, respectively.