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. 2005 Apr 22;33(7):2280–2289. doi: 10.1093/nar/gki515

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Splicing of mutants with a destabilized stem P3 is sensitive to StpA. (A) Schematic representation of investigated mutants. Notice that the mutation tdU912C causes a mismatch-destabilizing stem P3, whereas the double mutant tdA42G/U912C results in a stabilized P3 element which is also indicated by the T_m of the intron structure. (B) Splicing of mutants in stem P3 was assayed in the absence and presence of StpA and Cyt-18, respectively. Quantification of three independent experiments.