Table 2.
Reaction constants gained from cis-splicing assays using td wt and tdC865U mutant RNAs in the presence and absence of StpA or StpA-G126V, respectively
| Wt short exon construct | C865U short exon construct | |||||||
|---|---|---|---|---|---|---|---|---|
| Fast reacting | Slow reacting | Fast reacting | Slow reacting | |||||
| % | kobs (min−1) | % | kobs (min−1) | % | kobs (min−1) | % | kobs (min−1) | |
| No protein | 47 ± 4 | 0.2 ± 2 × 10−2 | 53 ± 4 | 0.2 × 10−2 ± 1 × 10−3 | 50 ± 4 | 0.2 ± 3 × 10−2 | 50 ± 4 | 0.7 × 10−2 ± 2 × 10−3 |
| 1.4 μM StpA | 60 ± 4 | 0.4 ± 8 × 10−2 | 40 ± 4 | 1.0 × 10−2 ± 3 × 10−3 | 50 ± 3 | 0.3 ± 6 × 10−2 | 50 ± 4 | 1.0 × 10−2 ± 2 × 10−3 |
| 1.4 μM G126V-StpA | 77 ± 6 | 0.4 ± 3 × 10−2 | 23 ± 4 | 0.8 × 10−2 ± 4 × 10−3 | 40 ± 6 | 0.2 ± 7 × 10−2 | 60 ± 4 | 0.9 × 10−2 ± 4 × 10−3 |
The fractions of fast or slow reacting RNA molecules with the corresponding reaction rates are indicated. The reaction constants were calculated using the equation given in Materials and Methods.