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. 2005 Apr 22;33(7):2310–2317. doi: 10.1093/nar/gki526

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Agarose gel electrophoresis showing the effect of radicicol on relaxation of the plasmid pBR322. Lane 1, supercoiled pBR322; lane 2, supercoiled pBR322 in the presence of radicicol (250 μM); lane 3, supercoiled pBR322 with Topo VI; lane 4, supercoiled pBR322 with Topo VI and DMSO; and lanes 5–10, supercoiled pBR322 with Topo VI in the presence of radicicol (25, 50, 100, 125, 200 and 250 μM, respectively). SC: negatively supercoiled pBR322 DNA; R: relaxed DNA; OC: open circular (nicked) pBR322 DNA.