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. 2005 Apr 22;33(7):2310–2317. doi: 10.1093/nar/gki526

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Agarose gel electrophoresis showing the effect of radicicol on supercoiling of the plasmid pBR322 by the DNA gyrase. Lane 1, control pBR322 negatively supercoiled; lane 2, relaxed pBR322 and 500 μM radicicol without gyrase; lane 3, relaxed pBR322 with gyrase; lanes 4 and 5, relaxed pBR322 with gyrase in the presence of 100 or 500 μM radicicol; and lane 6, relaxed pBR322 with gyrase in the presence of 100 μM novobiocin.