Skip to main content
. 2005 Apr 26;3(5):e159. doi: 10.1371/journal.pbio.0030159

Figure 7. Gene Expression Analysis upon Induction of Precocious or Isochronic ETS Signaling.

Figure 7

(A–H) Analysis of TrkC expression by in situ hybridization (A–D), or Calretinin (red) and LacZ (green) expression by immunohistochemistry (E–H), on E16.5 lumbar DRG of wild-type (A and E), TauEWS-Pea3/+ Isl1Cre/+ (B and F), Er81EWS-Pea3/− (C and G), and TauEWS-Pea3/+ PVCre/+ (D and H) embryos.

(I) Summary diagram illustrating deregulation of TrkC (red arrows, downregulation) and Calretinin (green arrows, upregulation) expression upon precocious (B and F) induction of EWS-Pea3 expression in DRG neurons (B and F; E10–E11, i.e., shortly after cell cycle exit, E9.5–E10). In contrast, activation of EWS-Pea3 from the endogenous Er81 locus (C and G; E12.5–E13) or via Cre recombinase expression from the PV locus activating late expression from the Tau locus (D and H; E14.5) does not interfere with the normal expression of TrkC and Calretinin (shown in grey).

Scale bar: (A–D), 65 μm; (E–H), 80 μm.