Table 2. Dissociation Constants of SynGUN4 Proteins for Deutero and Mg-Deutero.

Endogenous tryptophan fluorescence quenching (330 nm) was used to analyze each SynGUN4 mutant's dissociation constant for both Deutero and Mg-Deutero. More than 60 mutants were made, of which those listed above produces stable protein for binding analysis. Quenching data were fit to a single site model using GraphPad Prism version 4.0a for Macintosh (GraphPad Software, http://www.graphpad.com)