2.1.
Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
| Rabbit anti-GABA | Sigma-Aldrich | Cat#A2052; RRID: AB_477652 |
| Chicken anti-MAP2 | Abcam | Cat#ab5392; RRID: AB_2138153 |
| Rabbit anti-TBR1 | Abcam | Cat#ab31940; RRID: AB_2200219 |
| Alexa Fluor™ 488 goat anti-chicken IgG (H + L) | Invitrogen | Cat#A11039; RRID: AB_142924 |
| Alexa Fluor™ 647 goat anti-rabbit IgG (H + L) | Invitrogen | Cat#A21244; RRID: AB_2535812 |
| Hoechst | Invitrogen | Cat#H3569; RRID: AB_2651133 |
| Bacterial and virus strains | ||
| pLV-TetO-hNGN2-P2A-mCherry-T2A-Puro | This paper | N/A |
| pLV-TetO-hDLX2-P2A-hASCL1-T2A-Puro | This paper | N/A |
| pLV-hSyn-jRCaMP1b | This paper | N/A |
| Biological samples | ||
| Human iPSCs (Table 1) | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| ALDH5A1 mRNA | TriLink | Cat#ENST00000357578.8; TriLink, 1 mg scale, 5-Methoxyuridine, 1 mM Sodium Citrate, pH 6.4 |
| mRNA CleanCap® EGFP mRNA Media | TriLink | Cat#L-7601 |
| Lipofectamine MessengerMAX mRNA Transfection | Invitrogen | Cat#LMRNA001 |
| B27 Supplement | Life Technologies | Cat#17504–044 |
| N2 Supplement | Thermo Fisher | Cat#17502048 |
| Poly-D-lysine hydrobromide | Sigma-Aldrich | Cat#P6407 |
| Laminin | Life Technologies | Cat#23017–015 |
| Y-27632 | Cayman | Cat#10005583 |
| Doxycycline | Millipore | Cat#324385–1GM |
| Puromycin | Invitrogen | Cat#ant-pr-1 |
| BDNF | Peprotech | Cat#450–02 |
| GDNF | Peprotech | Cat#450–10 |
| NT3 | Peprotech | Cat#450–03 |
| Papain | Worthington | Cat##LK003178 |
| DNAseI | Worthington | Cat#LK003172 |
| Critical commercial assays | ||
| GHB kit | BUHLMANN Diagnostics Corp, Amherst, NH |
#CatKK-GHB-U |
| GABA kit | LDN, Nordhorn |
#CatBA E-2500 |
| Seahorse XF Cell Mito stress | Agilent | Cat# 103015–100 |
| CytoView MEA plates | Axion Biosystems |
#CatM768-tMEA-48B |
| Experimental models: Cell lines | ||
| Human iPSC-derived astrocytes | NCardia | Cat#M0605 |
| Oligonucleotides | ||
| Mycoplasma testing | CReM Boston | N/A |
| Myco280_CReM (5'- ACACCATGGGAGYTGGTAAT-3'); Myco279_CReM (5'- TTCWTCGACTTYCAGACCCAAGGCAT- 3') MGSO-5 (5'-TGCACCATCTGTCACTCYGTTAACCTC- 3') GPO-3 (5'- GGGAGCAAACAGGATTAGATACCCT- 3') |
University | |
| Recombinant DNA | ||
| pLV-TetO-hNGN2-P2A-mCherry-T2A-Puro | This paper | N/A |
| pLV-TetO-hDLX2-P2A-hASCL1-T2A-Puro | This paper | N/A |
| pLV-hSyn-jRCaMP1b | This paper | N/A |
| Software and algorithms | ||
| Fiji | Schindelin et al. (Schindelin et al., 2012) | https://imagej.net/software/fiji/ |
| FluoroSNNAP | Patel et al. (Patel et al., 2015) | https://www.seas.upenn.edu/~molneuro/software.html |
| Nikon NIS Elements | https://www.microscope.healthcare.nikon.com/products/software/nis-elements | |
| Gene ontology analysis | DAVID | https://david.ncifcrf.gov/summary.jsp |