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. Author manuscript; available in PMC: 2024 Dec 6.
Published in final edited form as: Neuron. 2023 Oct 2;111(23):3819–3836.e8. doi: 10.1016/j.neuron.2023.08.030

Figure 5: Igf2 knockout in dHC pericytes impairs novel object location and contextual fear memories.

Figure 5:

(A) Experimental schedule. DHC-Igf2/Peri-KO and dHC-Igf2/NoCre-TAM were assessed for (B) Total exploration time (left panel), and preference for the moved object (right panel) tested 4 hours after nOL training; nOL memory is expressed as mean percent preference ± s.e.m. for the moved object. (C) CFC training (left panel) and memory tested (right panel) at 1 and 7 days after training. CFC memory is expressed as mean percent freezing ± s.e.m. For B and C, n = 6–7 mice/group, 2 independent experiments. Dots on graphs represent the value for each mouse. Two-way RM ANOVA followed by Bonferroni’s post-hoc test. (D, E) qPCR analyses of Igf2 mRNA levels in dHC and primary somatosensory cortex (S1), and FACS-purified CD13+ (pericytes) and CD31+ (endothelial cells) from both regions. Data are expressed as fold change ± s.e.m. relative to the mRNA levels of dHC (dHC total) and S1 (S1 total); n = 4 mice/group per experiment, 4 independent experiments. Dots on graphs represent value for each experiment. Two-way ANOVA followed by Bonferroni’s post-hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001. Numeric values and detailed statistical analyses are reported in Table S1. Primers for qPCR in Table S3. Marker for endothelial cells: CD31; pericytes: CD13.