Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-PDGFRB | R&D Systems | Cat# AF1042 RRID:AB_2162633 |
| anti-Collagen1 | Thermo Fisher Scientific | Cat# 600-402-103 RRID:AB_2614800 |
| anti-CD13 | BD Pharmingen | Cat# 558744 RRID:AB_397101 |
| anti-COL1A1 | Invitrogen | Cat # MA5-43886 RRID:AB_2912818 |
| anti-CD31 | BD Pharmingen | Cat# 557355 RRID:AB_396660 |
| anti-IBA1 | Fujifilm Wako Chemicals | Cat# 019-19741 RRID:AB_839504 |
| anti-NeuN | Millipore Sigma | Cat# MAB377 RRID:AB_2298772 |
| anti-IGF2 receptor | Abcam | Cat# ab262713 RRID:AB_2936901 |
| anti-IGF2 | Abcam | Cat# ab9574 RRID:AB_308725 |
| anti-cation independent mannose 6 phosphate receptor | Abcam | Cat# ab124767 RRID:AB_10974087 |
| anti-Arc | Synaptic Systems | Cat# 156003 RRID:AB_887694 |
| anti-c-Fos | Cell Signaling Technology | Cat# 2250 RRID:AB_2247211 |
| anti-EGR1 | Cell Signaling Technology | Cat# 4153 RRID:AB_2097038 |
| Donkey anti-goat Alexa Fluor 647 | Invitrogen | Cat# A21447 RRID:AB_141844 |
| Goat anti-rabbit Alexa Fluor 647 | Invitrogen | Cat# A21244 RRID:AB_2535812 |
| Donkey anti-rat Alexa Fluor 488 | Invitrogen | Cat# A21208 RRID:AB_2535794 |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | Cat# A11034 RRID:AB_2576217 |
| Goat anti-rabbit Alexa Fluor 568 | Invitrogen | Cat# A11036 RRID:AB_10563566 |
| Dylight 594 Lycopersicon esculentum (Tomato) lectin | Vector Laboratories | Cat# DL-1177 RRID:AB_2336416 |
| Goat anti-mouse IRdye680LT | LI-Cor Bioscience | Cat# 926-68020 RRID:AB_10706161 |
| Goat anti-rabbitIRdye800CW | LI-Cor Bioscience | Cat# 926-32211 RRID:AB_621843 |
| anti-Actin | Santa Cruz Biotechnology | Cat# sc-47778 RRID:AB_626632 |
| Bacterial and virus strains | ||
| AAV8/hSyn-mCherry | Addgene | Cat# 114472 |
| AAV8/hSyn-hM4Di-mCherry | Addgene | Cat# 50475 |
| LV/Col1a1-Cre-EGFP | Vector Builder | |
| LV/Col1a1-EGFP | Vector Builder | |
| AAV8/hSyn-Cre-EGFP | Addgene | Cat# 105540 |
| AAV8/hSyn-EGFP | Addgene | Cat# 50465 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| 4-hydroxytamoxifen (4-OHT) | Sigma-Aldrich | Cat# H6278 |
| Pretreatment 4 solution | ACD | Cat# 320513 |
| RNAscope® 2.5 Fluorescent Multiplex Kit | ACD | Cat # 320293 |
| Compound 21 | HelloBio | Cat# HB6124 |
| ProLong™ Diamond Antifade Mountant | Life Technologies | Cat# P36961 |
| ProLong™ Diamond Antifade Mountant with DAPI | Life Technologies | Cat# P36962 |
| Ebioscience™ Intracellular Fixation & Permeabilization Buffer Set | Thermo Scientific | Cat# 88-8824-00 |
| RNeasy Universal Plus Mini Kit | Qiagen | Cat# 73404 |
| RNeasy Micro kit | Qiagen | Cat# 74004 |
| QuantiTect Reverse Transcription Kit | Qiagen | Cat# 205311 |
| iQ SYBR Green Supermix | Bio-Rad | Cat# 107-8882 |
| DAPI | Thermo Scientific | Cat# 62247 |
| Paraformaldehyde | Sigma | Cat# 158127 |
| Lipopolysaccharide (LPS) | Sigma | Cat# L6011 |
| Experimental models: Organisms/strains | ||
| Adult C57BL6 mice | Jackson Laboratories | Stock #000664 |
| Adult PDGFRB-P2A-CreERT2 mice | Jackson Laboratories | Stock #030201 |
| Adult Igf2-floxed mice | Kindly provided by Drs. Miguel Constancia and Ionel Sandovici, University of Cambridge | |
| Adult IGF2-tdTomato-H2B mice | Kindly provided by Cold Spring Harbor Laboratories (CSHL) | |
| Adult male Long Evans rats | Envigo | Order code: 140 |
| Software and algorithms | ||
| Image J | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Prism 7 | GraphPad Software Inc. | |
| EthoVision-XT13 | Noldus | |