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. Author manuscript; available in PMC: 2024 Feb 5.
Published in final edited form as: Neurobiol Dis. 2023 Nov 20;190:106359. doi: 10.1016/j.nbd.2023.106359

Fig. 4.

Fig. 4.

Effects of Dyrk1a copy number reduction on Dyrk1a mRNA expression in P6 male Ts65Dn mice. Quantitative polymerase chain reaction (qPCR) analysis was performed in mice of each genotype: euploid (Eu,Dyrk1a+/+), Trisomic (Ts,Dyrk1a+/+/+), and trisomic with 2 copies of Dyrk1a Ts,Dyrk1a+/+/−). Relative fold change was calculated using the 2−ΔCT comparative method relative to within-plate euploid means. Two directional hypotheses regarding the expression of Dyrk1a mRNA were tested separately for each brain region: a) Ts,Dyrk1a+/+/+ > Eu,Dyrk1a+/+ and b) Ts,Dyrk1a+/+/+ > Ts,Dyrk1a+/+/−, using one-tailed independent groups t-tests (alpha = 0.05). Data are shown as scatterplots with group means and plotted using a log 2 scale (euploid means = 1).