Figure 7. Comparison of therapeutic effect and T cell infiltration in TME after Nanodiscs and Listeria vaccine.
A) C57BL/6 mice were inoculated at s.c. flank with MC38 cancer cells on day 0. On days 10 and 17, animals were vaccinated with 107/100 μl/mouse Listeria-Adpgk intravenously (i.v.) or nanodisc-Adpgk/CpG (s.c.). Anti-PD-1 IgG was injected i.p. on days 11, 14, 18, and 21. For antitumor immune evaluation, the spleens were harvested on day 28 and processed for peptide stimulation and intracellular cytokine staining (ICS). B) Tumor growth was monitored. C) Adpgk-specific CD8a+ T cells were quantified by the tetramer staining among PBMCs, spleen, and tumor tissues. D) PBMCs, spleen, and tumor tissues were isolated and re-stimulated with Adpgk neoepitope, followed by intracellular cytokine staining. Data are presented as mean ± s.e.m. n = 10 for B) and n = 4–7 for C-D).