FIG. 1.

Sequences of the putative 5′ regulatory regions of the genes utilized as probes in Southwestern analysis. The flanking (5′ and 3′) primers used for probe amplification are indicated by dashed lines. (A) The previously described yeast phase-specific gene, yps-3; (B) yps 21:E-9; (C) the limited 5′ regulatory region of the α-tubulin gene (TUB1). Both yps genes are expressed in the yeast phase of the virulent strain G217B, but they are both silent in the yeast and mycelial phases of the attenuated Downs strain. α-Tubulin is expressed in both phases of the Downs and G217B strains, with fivefold-higher transcript levels detected in the mycelial phase (9). The transcriptional start sites, determined by S1 mapping of G217B poly(A)⁺ RNA, for each yps gene is shown (underlined), as well as the putative translational start sites (START) for all three sequences.