FIG. 6.

Southwestern analysis of nuclear extracts prepared from mycelial phase-to-yeast phase transforming cultures of G217B. Mycelial cells of strain G217B were induced to transform to the yeast phase at 37°C, and nuclear extracts were obtained from the terminal phases as well as 1 day, 3 days, 8 days, and 11 days following the shift-up in temperature. Proteins were electrophoresed in duplicate on SDS–10% polyacrylamide gels; half of the gel was stained to verify protein loading, and the remainder was transferred and evaluated for DNA binding. The results shown are from separate Southwestern blots with the 335-bp regulatory region of TUB1 (the α-tubulin gene) amplified from G217B and the 5′ regulatory region of yps 21:E-9 amplified from G217B.