Fig. 4. Restored the hematopoietic progenitor populations and lymphopoiesis of miR-9-1-deficient BM cells after transplantation.

BM cells from growth-retarded miR-9-1^−/− or wild-type control mice (CD45.2⁺) were transplanted into lethally irradiated CD45.1⁺ recipients. About eight weeks after transplantation, the recipients were analyzed. A The numbers of total BM cells, splenocytes and thymocytes the recipients that received BM cells from miR-9-1^−/− and controls mice. B The percentages and numbers of various CD45.2⁺ hematopoietic progenitors in the BM of the recipients that received BM cells from miR-9-1^−/− and controls mice. Percentages indicate LT-HSC, ST-HSC and MPP in the gated CD45.2⁺Lin⁻IL-7R⁻LSK population. C The percentages and numbers of B cells at the different developmental stages in the BM of the recipients that received BM cells from miR-9-1^−/− and controls mice. Percentages indicate pro/pre-, immature (IM) and mature (M) B cells in the gated CD45.2⁺ population. Data shown are obtained from 4 recipients that received control or miR-9-1^−/− BM cells. Each dot represents an individual mouse. Mean ± SD is shown. *, p < 0.05; **, p < 0.01.