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. 2023 Nov 27;38(2):340–350. doi: 10.1038/s41375-023-02093-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Overview of tumor-specific cell marker identification. For both LN (n = 9) and tumor cell-bearing PB (n = 12) samples, differentially expressed gene (DEG) analysis was performed to select genes whose expression was significantly elevated in tumor cells of two or more samples versus (vs.) all MNCs or normal T_FH cells (for only LN tumor cells) for all samples. Next, genes specifically expressed in tumor cells were selected by manual inspection using UMAP plots. B Representative FCM plots of PLS3 expression in PD1^bright CD4⁺ cells from PB of TFHL (AITL4, red) and PD1^dim CD4⁺ cells from PB of a healthy donor (HD, blue). C Representative images of PLS3 expression by immunohistochemical staining of FFPE samples from TFHL (AITL4, left) and B-cell lymphoma (B03, right). White triangles indicate tumor cells expressing PLS3 on the cell membrane surface. Images were scanned using the NanoZoomer (2.0HT, Hamamatsu Photonics, Shizuoka, Japan) and acquired at ×10 and ×40 with the NDP.view.2 software (v2.9.29). FL, follicular lymphoma; G, grade of FL according to the 4th edition of the World Health Organization classification [1]; Scale bar, 250 μm (left) and 50 μm (right).