TABLE 1.

Sequence of unpredictable chronic mild stress (UCMS) procedures. UCMS stressors included a two-hour restraint in a plastic tube (Restraint), a 15-min exposure to fox urine (Fox), a 5-min swim in cool (15°C) water (Cool Swim), overnight exposure to soiled bedding from breeder cages (Soiled), 5-min exposure to a cool air stream from a hair dryer (Dryer), 3 to 8 h exposed to a flooded cage (Flood), and 2–3 h exposed to a cage tilt at 45° (Tilt). UCMS began on a Monday, and stressors were delivered Sunday through Monday with Saturdays off. Stressors, excepting overnight exposure to soiled bedding, were administered randomly from 8:00 to 17:00, and rats did not see the same stressors twice in a row. On the last Sunday of the UCMS period rats had a third EchoMRI analysis, and UCMS concluded with an overnight fast and an intraperitoneal glucose tolerance test (IPGTT).

Week	Sunday	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday
1		Restraint	Fox	Cool swim	Soiled	Dryer
2	Flood	Soiled	Cool swim	Tilt	Flood	Fox
3	Cool swim	Dryer	Restraint	Soiled	Tilt	Flood
4	Soiled	Cool Swim	Fox	Flood	Dryer	Restraint
5	EchoMRI	Dryer	Fast	IPGTT