Table 1.

Evaluation of sperm quality attributes in different selenium nanoparticles supplemented and without supplemented groups at different hours of preservation.

Parameters	Hours	Control (without supplementation)	T1 (0.5 μL ml−1)	T2 (1.0 μL ml−1)	T3 (2.0 μL ml−1)	p-value
Total sperm motility (%)	0	87.4A ± 0.45	88.37A ± 0.47	88.47A ± 0.46	87.75A ± 0.43	0.340NS
	24	75.10cB ± 0.57	77.30bB ± 0.56	79.60aB ± 0.47	75.52cB ± 0.56	0.000**
	48	63.00cC ± 0.64	67.25bC ± 0.56	71.25aC ± 0.55	64.00cC ± 0.69	0.000**
	72	51.62cD ± 0.60	56.75bD ± 0.60	61.12aD ± 0.52	53.25cD ± 0.70	0.000**
	96	40.75dE ± 0.52	45.75bE ± 0.52	50.12aE ± 0.48	42.75cE ± 0.53	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**
Live sperm (%)	0	86.12A ± 0.41	86.72 A ± 0.37	87.05 A ± 0.28	86.22 A ± 0.43	0.279NS
	24	79.25 dB ± 0.40	81.40bB ± 0.31	82.47aB ± 0.28	80.22cB ± 0.34	0.000**
	48	69.40cC ± 0.56	72.17bC ± 0.46	74.37aC ± 0.42	70.52cC ± 0.51	0.000**
	72	60.05cD ± 0.71	62.95bD ± 0.62	65.37aD ± 0.58	61.57bcD ± 0.60	0.000**
	96	50.57cE ± 0.76	54.25bE ± 0.64	56.82 aE ± 0.63	52.82bE ± 0.68	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**
Plasma membrane integrity (%)	0	63.60 A ± 0.46	63.92A ± 0.52	64.62A ± 0.47	63.70A ± 0.49	0.451NS
	24	52.30bB ± 0.53	53.57abB ± 0.53	54.95aB ± 0.55	52.70bB ± 0.45	0.002**
	48	42.50cC ± 0.61	44.37bC ± 0.62	46.10aC ± 0.61	43.25bcC ± 0.57	0.000**
	72	33.92cD ± 0.60	36.20bD ± 0.65	38.07aD ± 0.67	35.07bcD ± 0.61	0.000**
	96	26.15cE ± 0.57	28.32bE ± 0.58	30.12aE ± 0.61	27.15bcE ± 0.57	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**
Acrosomal integrity (%)	0	92.82A ± 0.33	93.20A ± 0.33	93.27A ± 0.38	92.92A ± 0.31	0.754NS
	24	82.85cB ± 0.42	84.22abB ± 0.38	85.15aB ± 0.37	83.07bcB ± 0.39	0.000**
	48	72.80bC ± 0.51	74.95aC ± 0.46	76.17aC ± 0.42	73.65bC ± 0.44	0.000**
	72	63.45cD ± 0.64	65.87abD ± 0.60	67.07aD ± 0.62	64.60bcD ± 0.62	0.000**
	96	53.75cE ± 0.70	56.27abE ± 0.66	57.92aE ± 0.67	55.10bcE ± 0.70	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**
Sperm DNA integrity (%)	0	99.70A ± 0.07	99.77A ± 0.06	99.85A ± 0.05	99.80A ± 0.06	0.439NS
	24	99.60AB ± 0.07	99.67 AB ± 0.07	99.75A ± 0.06	99.70 AB ± 0.07	0.547NS
	48	99.40bB ± 0.07	99.52 abB ± 0.07	99.70aA ± 0.07	99.50abB ± 0.08	0.057*
	72	98.95cC ± 0.10	99.25 abC ± 0.08	99.42aB ± 0.09	99.05bcC ± 0.08	0.002**
	96	98.22cD ± 0.12	98.80aD ± 0.08	98.97aC ± 0.06	98.50bD ± 0.10	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**
Mitochondrial membrane potential of sperm cell (%)	0	85.77bA ± 0.30	86.25abA ± 0.34	86.85aA ± 0.29	85.75bA ± 0.30	0.046*
	24	79.72 dB ± 0.23	81.52bB ± 0.22	82.70aB ± 0.16	80.35cB ± 0.15	0.000**
	48	72.62dC ± 0.29	74.77bC ± 0.31	76.17aC ± 0.21	73.65cC ± 0.29	0.000**
	72	65.57cD ± 0.29	67.50bD ± 0.31	69.40aD ± 0.28	66.67bD ± 0.31	0.000**
	96	57.55dE ± 0.31	60.07bE ± 0.26	62.15aE ± 0.27	59.17cE ± 0.20	0.000**
	p-value	0.000**	0.000**	0.000**	0.000**

T1: Supplemented with 0.5 μg ml−1 of Se-NPs; T2: supplemented with 1 μg ml−1 of Se-NPs; T3: supplemented with 2 μg ml−1 of Se-NPs.

**p < 0.01; *p < 0.05; ^NSNon-significant.

Means with different superscripts in a row (a, b, c, d) differed significantly.

Means with different superscripts in a column (A, B, C, D, E) differed significantly.