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(A) Total overall number of observations of “hypersensitive to touch” and/or “vocalization”; (B) Total overall number of rats noted with observations of “hypersensitive to touch” and/or “vocalization”. *Significantly different from group A (pair housed, handled weekly by encircling the torso) (P < 0.01). +Significantly different from group C (singly housed, handled weekly by encircling the torso) (P < 0.05). #Males significantly different from females (P < 0.01).
