Table 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Description | Purpose | Direct background strain | Source |
|---|---|---|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Wild-type | Background strain | - | Euroscarf |
| ΔEFM4 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 EFM4::kanMX4 | EFM4 knockout | Background strain | BY4741 | Euroscarf |
| EFM4-His | EFM4::EFM4- 6xHis (URA3) | -WT EFM4 with C-terminal 6xHis-tag; downstream URA3 | Control for EFM4 mutant strains | BY4741 | This study |
| EFM4-F210A-His | EFM4::EFM4-F210A-6xHis (URA3) | -EFM4 with F210A mutation and C-terminal 6xHis-tag; downstream URA3 | Efm4 in vivo mutagenesis | BY4741 | This study |
| EFM4-F212A-His | EFM4::EFM4-F212A-6xHis (URA3) | -EFM4 with F212A mutation and C-terminal 6xHis-tag; downstream URA3 | Efm4 in vivo mutagenesis | BY4741 | This study |
| ΔEFM4 pD1204-eEF1A1 | EFM4::kanMX4, pD1204-eEF1A1 | -EFM4 knockout -Human eEF1A1 under galactose-inducible promoter |
Purification of WT eEF1A1 for eEF1A-KMT2 methylation assays | ΔEFM4 | This study |
| ΔEFM4 pD1204-eEF1A2 | EFM4::kanMX4, pD1204-eEF1A2 | -EFM4 knockout -Human eEF1A2 under galactose-inducible promoter |
Purification of WT eEF1A2 for eEF1A-KMT2 methylation assays | ΔEFM4 | This study |
| TEF1-His | TEF1::TEF1-6xHis (URA3) | -WT TEF1 with C-terminal 6xHis-tag; downstream URA3 | Control for eEF1A mutant strains | BY4741 | This study |
| TEF1-His ΔTEF2 | TEF1::TEF1-6xHis (URA3), TEF2::natNT2 | -TEF1 with C-terminal 6xHis-tag; downstream URA3 -TEF2 knockout |
Purification of eEF1A for phospho-enrichment | TEF1-His | This study |
| TEF1-S314A-His | TEF1::TEF1-S314A-6xHis (URA3) | -TEF1 with S314A mutation and C-terminal 6xHis-tag; downstream URA3 | eEF1A in vivo mutagenesis | BY4741 | This study |
| TEF1-S314D-His | TEF1::TEF1-S314D-6xHis (URA3) | -TEF1 with S314D mutation and C-terminal 6xHis-tag; downstream URA3 | eEF1A in vivo mutagenesis | BY4741 | This study |
| ΔEFM4 TEF1-His | EFM4::kanMX4, TEF1::TEF1-6xHis (URA3) | -EFM4 knockout -WT TEF1 with C-terminal 6xHis-tag; downstream URA3 |
Purification of WT eEF1A for Efm4 methylation assays | ΔEFM4 | This study |
| ΔEFM4 TEF1-S314A-His | EFM4::kanMX4, TEF1::TEF1-S314A-6xHis (URA3) | -EFM4 knockout -TEF1 with S314A mutation and C-terminal 6xHis-tag, downstream URA3 |
Purification of S314A eEF1A for Efm4 methylation assays | ΔEFM4 | This study |
| ΔEFM4 TEF1-S314D-His | EFM4::kanMX4, TEF1::TEF1-S314D-6xHis (URA3) | -EFM4 knockout -TEF1 with S314D mutation and C-terminal 6xHis-tag, downstream URA3 |
Purification of S314D eEF1A for Efm4 methylation assays | ΔEFM4 | This study |