Figure 2.

RGS14 ameliorates lipid accumulation in hepatocytes. (A) Protein levels of Rgs14 in primary mouse hepatocytes transfected with adenovirus loading Rgs14 (Ad-Rgs14) or vector (Ad-Vec), n = 3 per group. (B) Representative images (n = 3 independent experiments) and quantification (n = 7 high power fields per group) of Nile Red staining of Rgs14-overexpressed primary mouse hepatocytes under the stimulation of PO or BSA treatment for 24 h. Scale bar, 10 um. (C) TG contents in Rgs14-overexpressed primary hepatocytes under PO treatment (n = 3 independent experiments). (D–F) mRNA levels of fatty acid uptake and synthesis genes (D), fatty acid oxidation genes (E) and inflammation genes (F) in Rgs14-overexpressing primary mouse hepatocytes treated with PO at 12 h, β-Actin was used as internal control, n = 3 independent experiments. (G) Protein expression of Rgs14 protein expression in primary mouse hepatocytes transfected with each indicated adenovirus (Ad-ShNc or Ad-ShRgs14), β-Actin was served as control, n = 3 independent experiments. (H) Representative images (n = 3 independent experiments) and quantification (n = 7 high power fields per group) of Nile Red staining of Rgs14 knockdown (ShRgs14) in primary mouse hepatocytes under PO treatment for 24 h. Scale bar, 10 um. (I) Intracellular TG content in the primary mouse hepatocytes of Rgs14 knockdown after PO treatment 24 h (n = 3 independent experiments). (J–L) The gene expression of fatty acid uptake and synthesis genes (J), fatty acid oxidation genes (K) and inflammation genes (L) in Rgs14 knock-down primary mouse hepatocytes under PO treatment for 12 h (n = 3 independent experiments). One-way ANOVA analysis was used. ∗P < 0.05, ∗∗P < 0.01, n.s. indicates no significance between the two indicated groups. All data are shown as the mean ± s.d. Abbreviations: BSA, Bovine Serum Albumin; PO, 0.5 mM Palmitic Acid and 1 mM Oleic Acid. Ad, Adenovirus; TG, Triglyceride.