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. 2024 Jan 17;80:101882. doi: 10.1016/j.molmet.2024.101882

Figure 4.

Figure 4

RGS14 depends on GDI activity to ameliorate hepatocytes lipid accumulation. (A) Protein levels of Rgs14 and Rgs14 mutants in primary mouse hepatocytes transfected with each indicated adenovirus, n = 3 independent experiments. (B) Representative images (n = 3 independent experiments) and quantification (n = 7 high power fields per group) of Nile Red staining of Rgs14 or Rgs14 mutants overexpressed primary mouse hepatocytes under PO treatment 24 h. Scale bar, 10 um. (C) TG contents in Rgs14 or Rgs14 mutants overexpressed primary mice hepatocytes under PO treatment (n = 3 independent experiments). (D–F) mRNA levels of fatty acid uptake and synthesis genes (D), fatty acid oxidation genes (E) and inflammation genes (F) in each indicated adenovirus infected primary mouse hepatocytes stimulated by PO for 24 h (n = 3 independent experiments). One-way ANOVA analysis was used. ∗P < 0.05, ∗∗P < 0.01, n.s. indicates no significance between the two indicated groups. All data are shown as the mean ± s.d. Abbreviations: PO, 0.5 mM Palmitic Acid and 1 mM Oleic Acid; TG, Triglyceride; Ad, Adenovirus.