Skip to main content
. 2024 Jan 17;80:101882. doi: 10.1016/j.molmet.2024.101882

Figure 5.

Figure 5

RGS14 depends on GDI activity to relieve HFHC-induced NASH. (A) Experimental schematic design to assess the functions of RGS14 in GDI activity on HFHC-induced NASH. (B) Protein expression of Rgs14 or Rgs14(Q518A) in male mice liver tissues that were injected with the pt3-Vec, pt3-Rgs14 and pt3-Rgs14(M) plasmids by Sleeping Beauty transposon system. (C) Body weight in Rgs14, Rgs14(M) and Vec mice fed with NC and HFHC diet from 0 to 16 weeks (n = 8 mice per group). (D) Liver weight and Liver weight to body weight ratio of Rgs14, Rgs14(M) and Vec mice that were fed with NC and HFHC for 16 weeks (n = 8 mice per group). (E) Representative images of H&E and Oil red O staining and their NAFLD Activity Score/Oil red O-positive area of liver sections in Rgs14, Rgs14(M) and Vec mice at 16 weeks after NC and HFHC treatment, n = 5 mice in each group, Scale bar, 100 um. (F) Serum TG and TC contents of Rgs14, Rgs14(M) and Vec mice fed with NC or HFHC (NC = 6 mice per group, HFHC = 7 mice per group). (G) TG, TC and NEFA contents of Rgs14, Rgs14(M) and Vec mice in hepatic samples after 16 weeks feeding with NC or HFHC (NC = 7 mice per group, HFHC = 9 mice per group). (H, I) The mRNA levels of fatty acid uptake and synthesis genes (H) and fatty acid oxidation genes (I) in liver tissues in Rgs14, Rgs14(M) and Vec HFHC mice (n = 7 mice per group). (J, K) Representative images of CD11b-positive cell staining and PSR of liver sections in Rgs14, Rgs14(M) and Vec HFHC mice (n = 5 mice per group), Scale bar, 100 um. (L, M) mRNA levels of inflammation genes (L) and fibrosis genes (M) in liver tissues in Rgs14, Rgs14(M) and Vec HFHC mice (n = 7 mice per group). (N) AST and ALT levels of Rgs14, Rgs14(M) and Vec mice after 16 weeks feeding with NC or HFHC (NC = 6 mice per group, HFHC = 7 mice per group). One-way ANOVA analysis was used. ∗P < 0.05, ∗∗P < 0.01, n.s. indicates no significance between the two indicated groups. All data are shown as the mean ± s.d. Abbreviations: GDI, GDP dissociation inhibitor; Rgs14(M), Rgs14(Q518A); H&E, Hematoxylin and Eosin; TG, Triglyceride; TC, Total Cholesterol; NEFA, Non-esterified fatty acid; PSR, Picrate Sirius Red staining; ALT, Alanine aminotransferase; AST, Aspartate aminotransferase.