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. 2024 Jan 10;80:101876. doi: 10.1016/j.molmet.2024.101876

Figure 4.

Figure 4

NF1 deficiency increases intracellular lipid pools. A) NF1 deficiency alters the ER + breast cancer metabolome. PCA analysis of NF1-EV and NF1-deficient NF1-45 MCF7 LC-MS data (n = 1, 3 technical replicates per biological replicate). B) Schematic of carbon flow from [U–13C]-glucose. C) NF1 deficiency decreases glucose incorporation into TCA intermediates. Relative metabolite enrichment compared to M+6 [U–13C]-glucose (n = 1, 3 technical replicates per biological replicate). D) Schematic of carbon flow from [U–13C]-glutamine. E) NF1 deficiency increases glutamine incorporation into TCA intermediates. Relative metabolite enrichment compared to M+5 [U–13C]-glutamine (n = 1, 3 technical replicates per biological replicate). F) NF1 deficiency increases TCA and REDOX intermediates and intracellular lipid species. Volcano plots describing aqueous and organic metabolite pool sizes between NF1-45 and NF1-EV control (n = 1, 3 technical replicates per biological replicate). The shape represents the geometric mean of the three technical replicates. G) NF1 deficiency increases total intracellular lipids. Boxplot of the sum of ion counts normalized to the mean ion count of the NF1-EV control within each lipid class. The line represents the geometric mean of the three technical replicates and the box boundaries at the 1st and 3rd quartile boundaries (n = 1, 3 technical replicates per biological replicate).