TABLE 1.
Strains used in this study
EAggEC or CDEC strain | Serotype | AA probea | Hly presence or absencec | PCR band (hlyA)d | LDH releasee | IL-1β releasee |
---|---|---|---|---|---|---|
O42 | O44:H18 | + | − | − | − | − |
17-2 | O3:H2 | + | + | + | + | + |
JM221 | O92:H33 | + | − | − | − | − |
69H77-1 | − | − | − | − | − | |
52H72.2 | − | NDb | ND | − | ND | |
DS244R3 | + | − | ND | − | ND | |
WC216-1-1 | R:H27 | + | − | ND | − | ND |
WC201-1-4 | O81:NM | − | + | − | − | ND |
H32-1 | − | − | ND | − | − | |
Thai501-1-1 | Rough | − | + | − | − | ND |
DS65-R3 | − | − | ND | − | ND | |
81H194-2 | + | − | ND | − | ND | |
54H-191-1 | + | − | ND | − | ND | |
697 (WRAIR) | ND | + | + | + | + | |
55-3 Hly+ (CDEC) | O6:H− | ND | + (two copies) | + | + | + |
55-3 Hly− (CDEC) | ND | − | − | − | − |
AA probe refers to a specific DNA probe present in some but not all EAggEC strains (see references 3 and 7).
ND, not determined.
Hly determined on blood agar plates.
hlyA determined by PCR amplification with hlyA primers.
LDH activity and IL-1β release were determined with both HMDM and J774 cells.